normal gastric epithelial cells  (Procell Inc)

Journal: Cell Death & Disease

Article Title: ALDH3A2 negatively orchestrates gastric cancer progression through a synergistic induction of ferroptosis and ferroptosis-driven macrophage reprogramming

doi: 10.1038/s41419-025-08364-8

Figure Lengend Snippet: A Volcano plot of differentially expressed genes between gastric cancer and adjacent non-tumor tissues. B Venn diagram showing the overlap of GC-related differentially expressed genes (DEGs) with ferroptosis-related genes (FerrDb) and mitochondrial dysfunction-related genes (MitoProteome). Prognostic gene screening based on overall survival using Univariate Cox regression ( C ), LASSO Cox analysis ( D , E ), Boruta ( F ), and Random Forest ( G ). H Upset plot showing ALDH3A2, PDK4, and GJA1 as common candidate genes identified across all machine learning methods. I Immunohistochemistry images of ALDH3A2, PDK4, and GJA1 proteins from the Human Protein Atlas. J Relative mRNA expression of ALDH3A2 in GES-1, SGC7901, MGC803, HGC27, and MKN45 cells assessed by qRT-PCR (n = 4). K Protein expression of ALDH3A2 in GES-1, SGC7901, MGC803, HGC27, and MKN45 cells were assessed by immunoblotting (n = 4). L Relative mRNA expression of PDK4 and GJA1 in GES-1, HGC27, and MGC803 cells assessed by qRT-PCR (n = 4). Statistical significance was determined by an unpaired Student’s t-test. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant.

Article Snippet: Human normal gastric mucosal epithelial cells GES-1, gastric cancer cell lines MGC803, HGC27, SGC7901, MKN45, and human embryonic kidney cells (293 T) used in this study were all sourced from Procell (Wuhan, China).

Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR, Western Blot

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: Identification and validation of hub genes in H. pylori-associated stomach adenocarcinoma (STAD). A Differentially expressed genes (DEGs) identified from GSE13911 and GSE54129 datasets. B Venn diagram showing 234 overlapping DEGs shared between the two datasets. C Protein–protein interaction (PPI) network constructed from common DEGs using the STRING database. D Top hub genes ranked by degree centrality using the CytoHubba plugin in Cytoscape. E RT-qPCR validation of hub gene expression in eight gastric cancer cell lines versus five normal gastric epithelial cell lines. F Receiver operating characteristic (ROC) curve analysis showing diagnostic performance (AUC values) of THBS2, CTNNB1, COL4A1, and E2F3 in distinguishing cancerous from normal tissues. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Construct, Quantitative RT-PCR, Gene Expression, Diagnostic Assay

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: Epigenetic regulation and pathway activity of hub genes in STAD. A Promoter methylation levels of THBS2, CTNNB1, COL4A1, and E2F3 in tumor vs. normal tissues using UALCAN database. B Correlation analysis between promoter methylation and gene expression using GSCA database. C Pathway activity profiles showing functional enrichment: THBS2, COL4A1, and E2F3 activate epithelial–mesenchymal transition (EMT) pathways; CTNNB1 is associated with suppression of hormone receptor pathways; E2F3 additionally activates cell cycle-related signalling. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Activity Assay, Methylation, Gene Expression, Functional Assay

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: miRNA–mRNA regulatory network and diagnostic value of miRNAs targeting hub genes in STAD. A miRNA–mRNA interaction network constructed using the miRNet database. B Expression analysis of key miRNAs (hsa-miR-15b and hsa-miR-9-2) in STAD vs. normal tissues using UALCAN database. C Kaplan–Meier survival analysis showing no significant association between the expression of hsa-miR-9-3p and hsa-miR-9-5p and overall survival in STAD patients. D RT-qPCR validation of hsa-miR-9-3p and hsa-miR-9-5p expression in eight GC cell lines and five normal gastric epithelial cell lines, confirming their upregulation in cancer. E ROC analysis demonstrating the diagnostic accuracy of hsa-miR-9-3p (AUC = 0.81) and hsa-miR-9-5p (AUC = 0.82) for distinguishing STAD cells from normal counterparts. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Diagnostic Assay, Construct, Expressing, Quantitative RT-PCR, Biomarker Discovery